Article:Oxidative Stress-Related Mechanisms and Antioxidant Therapy in Diabetic Retinopathy (5317113)

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This page is the ScienceSource HTML version of the scholarly article described at https://www.wikidata.org/wiki/Q29248994. Its title is Oxidative Stress-Related Mechanisms and Antioxidant Therapy in Diabetic Retinopathy and the publication date was 2017-02-06. The initial author is Cheng Li.

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Journal Information

Title: Oxidative Medicine and Cellular Longevity

Oxidative Stress-Related Mechanisms and Antioxidant Therapy in Diabetic Retinopathy

  • Cheng Li
  • Xiao Miao
  • Fengsheng Li
  • Shudong Wang
  • Quan Liu
  • Yonggang Wang
  • Jian Sun

1The First Hospital of Jilin University, Changchun 130021, China

2The Second Hospital of Jilin University, Changchun 130041, China

3General Hospital of the PLA Rocket Force, Beijing 100088, China

Publication date (ppub): /2017

Publication date (epub): 2/2017

Abstract

Diabetic retinopathy (DR) is one of the most common microvascular complications of diabetes and is the leading cause of blindness in young adults. Oxidative stress has been implicated as a critical cause of DR. Metabolic abnormalities induced by high-glucose levels are involved in the development of DR and appear to be influenced by oxidative stress. The imbalance between reactive oxygen species (ROS) production and the antioxidant defense system activates several oxidative stress-related mechanisms that promote the pathogenesis of DR. The damage caused by oxidative stress persists for a considerable time, even after the blood glucose concentration has returned to a normal level. Animal experiments have proved that the use of antioxidants is a beneficial therapeutic strategy for the treatment of DR, but more data are required from clinical trials. The aims of this review are to highlight the improvements to our understanding of the oxidative stress-related mechanisms underlying the development of DR and provide a summary of the main antioxidant therapy strategies used to treat the disease.

Paper

1. Introduction

Diabetes, a chronic metabolic disease, includes types I (lack of insulin) and II (insulin resistance). Globally, approximately 415 million people suffer from diabetes, and one person dies every six seconds with this disease. Moreover, a new patient is diagnosed every two seconds [[1]]. Diabetic retinopathy (DR), a sight-threatening microvasculature impairment, is one of the complications that seriously threaten the life of diabetic patients. DR is acknowledged as the main cause of blindness among working-age adults throughout the world [[2]]. In 2010, there were 126.6 million patients with DR, and it is predicted that this figure will increase to 191.0 million by 2030; consequently, the number of those with sight-threatening DR will increase from 37.3 million to 56.3 million during this period [[3]].

The development of DR is associated with sustained metabolic disorders caused by hyperglycemia and increased levels of inflammatory cytokines in the blood. Systemic inflammation caused by these metabolic alternations leads to hemodynamic changes, blood-retinal barrier (BRB) damage, the leakage of retinal microvessels and edema, a gradual thickening of the retinal vascular basement membrane, and a loss of pericytes. These lesions continue with the progression of diabetes. During the first few years of diabetes, patients may barely be aware of, or exhibit, retinal injury. However, the obvious symptoms of DR will be present in nearly all patients with type I diabetes for 20 years and in nearly 80 percent of those with type II diabetes for the same duration [[4]]. Therefore, an understanding of the mechanisms by which these pathogenic alterations are induced will improve the development of effective strategies to prevent and postpone the progression of DR.

Oxidative stress is thought to be one of the crucial factors in the pathogenesis of DR. Abnormal metabolism induced by hyperglycemia can result in the overproduction of free radicals such as hydroxyl and superoxide radicals, which are known as reactive oxygen species (ROS) [[5]]. The accumulation of ROS can lead to oxidative stress, which damages the tissue in and around retinal vessels, ultimately resulting in DR. It is well known that hyperglycemia can cause vascular damage through four classical mechanisms: increased polyol pathway flux; increased intracellular formation of advanced glycation end-products (AGEs) and expression of the receptor for AGEs; activation of the protein kinase C (PKC) pathway; and activation of the hexosamine pathway [[6]]. Moreover, the nuclear factor, erythroid 2 like 2- (NFE2L2-) related pathway (NFE2L2 is also known as Nrf2), GTP-binding proteins, and epigenetic modifications have also attracted increasing attention in recent years [[7][9]]. All these pathways are associated with the overproduction of ROS. In particular, oxidative stress induced by epigenetic modifications can persist for a considerable time, even after the blood glucose concentration returns to normal; this is called “metabolic memory” [[10]]. Thus, scavenging and/or reducing ROS production by these pathways may provide new therapeutic strategies. At present, multiple antioxidants have been used in clinical trials, but the results are not clear. In this review, we summarize several of the mechanisms induced by oxidative stress that cause DR and focus on the latest research into the treatment of the disease using antioxidants.

2. Diabetes-Induced Production of ROS

Oxidative stress can induce damage to target organs such as the retina [[11]]. The production of ROS mainly depends on two factors: (a) mitochondrial oxidative phosphorylation [[12]] and (b) the nicotinamide adenine dinucleotide phosphate- (NADPH-) oxidase (Nox) system [[13]]. Mitochondria are the major endogenous source of ROS and can utilize 95% of the available oxygen to produce ATP (Figure 1). Normally, ~2% of oxygen enters the electron transport chain and is subsequently oxidized to superoxides such as O2− and hydrogen peroxide. In diabetes, the uncoupling of mitochondrial electron transport leads to excessive superoxide production, which may stimulate several abnormal biochemical metabolic pathways, such as the polyol, PKC, and AGE pathways [[14]]. It has been reported that the levels of transcription of mitochondrial DNA-encoded NADH dehydrogenase 1 and 6 of complex I and cytochrome b of complex III are subnormal in the retinas of diabetic patients [[15], [16]], which contributes to the development of DR. Moreover, superoxides can also react with nitric oxide (NO) to form peroxynitrite. Peroxynitrite can oxidize small-molecule antioxidants such as glutathione (GSH), cysteine, and tetrahydrobiopterin [[17]], resulting in cytotoxicity due to lipid peroxidation, inactivation of enzymes by oxidation of sulfhydryl moieties and nitration of tyrosine, and damage to DNA [[14], [18], [19]].

The Nox system can generate ROS and catalyze molecular oxygen to produce superoxides and/or hydrogen peroxide by accepting electrons from NADPH and transporting them to molecular oxygen. The Nox system is a major source of oxidative stress in the vascular system and can be divided into different isoforms. Vascular Nox comprises membrane-bound subunits (Nox protein and p22phox) and cytosolic subunits (p47phox, p67phox, and Rac). Isoforms Nox 1, Nox 2, and Nox 4 are highly expressed in the vascular system [[20]]. The activity of Nox 2 increases in the retinas of diabetic mice. This increase is associated with increased production of ROS and the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular endothelial growth factor (VEGF), which can be inhibited by deletion of the Nox 2 gene [[21]]. A recent study has shown that Nox 1 expression and activity increase in the microvascular endothelial cells in the brains of diabetic mice [[22]]. Similar findings have also been reported in kidney vessels, coronary microvessels, and aortic endothelial cells [[23], [24]].

3. Hyperglycemia-Induced Pathological Changes in DR

The retina is the most metabolically active tissue in the body and is therefore easily affected by diabetes. Sustained hyperglycemia damages the microvasculature of the retina resulting in hemodynamic changes, thickening of the basement membrane, loss of pericytes, and BRB dysfunction [[25], [26]]. These abnormalities cause retinal ischemia and the release of proinflammatory and proangiogenic factors, which lead to inflammation and angiogenesis. The tight junctions between retinal pigment epithelial (RPE) cells constitute the BRB, which protects the retina from abnormal effusion from the choroid. Hyperglycemia produces elevated levels of methylglyoxal due to increased glycolysis. Methylglyoxal activates matrix metalloproteinases, which may facilitate an increase in vascular permeability by a mechanism involving the proteolytic degradation of tight junction proteins such as occludin. Fluid leakage therefore increases in the surrounding retinal tissue, resulting in macular edema and visual loss [[27]].

The retina is susceptible to oxidative stress owing to its hypermetabolic state [[28]], and the levels of oxidative stress markers are related to the severity of DR [[29]]. Experiments have demonstrated that the degeneration of retinal capillaries in diabetes can be reduced by antioxidant therapy via activation of caspase-3 and nuclear factor-κB (NF-κB), indicating that oxidative stress plays an important role in retinal capillary apoptosis [[30], [31]]. The dysfunction of rod and cone photoreceptors is thought to contribute to the development of retinal hypoxia and neovascularization [[32], [33]]. Patients with diabetes and the outer retinal degenerative disorder retinitis pigmentosa have a reduced risk of the development of preproliferative DR [[34], [35]]. It has been demonstrated that the retinas of rhodopsin knockout mice exhibit vascular attenuation in the capillary bed; however, in the retina of rhodopsin knockout mice with diabetes, it appears to have fewer vascular attenuation than that of nondiabetic counterparts [[36]]. The reason may be that the loss of rod photoreceptors during retinitis pigmentosa leads to a net reduction in oxygen usage by the retina. Thus, it may offset the exacerbation of hypoxia during DR, thereby protecting the microvasculature from pathogenic change. Furthermore, hyperglycemia-induced endothelial injury can produce reactive nitrogen species through the activation of arginase, leading to disruption of the nitroso-redox balance [[37]]. Finally, increased levels of nitrogen species, including NO and peroxynitrite, promote leukocyte adhesion to retinal vessels, BRB breakdown, and RPE damage [[38], [39]].

4. Role of Oxidative Stress in Hyperglycemia-Induced Pathological Changes in DR

4.1. General Mechanisms Underlying DR

As shown in Figure 2, four classical mechanisms are involved in the pathology of DR: increased polyol pathway flux, PKC pathway activity, accumulation of AGEs, and activation of the hexosamine pathway.

Hyperglycemia induces the activation of the polyol pathway and increases intracellular glucose flux [[40]]. Excess intracellular glucose is converted to sorbitol by aldose reductase, and sorbitol is oxidized to fructose by sorbitol dehydrogenase. During the reaction, these two enzymes can oxidize NADPH to NADP+and convert NAD+ to NADH, respectively. As a result, NADPH is consumed, the ratio of NADH/NAD+ increases, and the synthesis of NO and GSH decreases, all of which are important to maintaining the redox balance. Moreover, the accumulation of sorbitol is associated with basement membrane thickening, endothelial cell death, and pericyte apoptosis [[41], [42]]. Thus, activation of the polyol pathway plays a crucial role in the pathogenesis of DR.

PKC is a serine/threonine-related protein kinase; it mainly has three isoforms that are involved in diabetes: PKC-β, PKC-δ, and PKC-ζ. Hyperglycemia primarily activates PKC-β, which is associated with neovascularization. PKC-β can increase the expression of VEGF [[43]]. Retinal ischemia induces the overexpression of PKC-β in transgenic mice, whereas a lack of PKC-β reduces angiogenesis [[44]]. PKC-δ can be activated by aldose reductase [[45]], and the PKC-δ/p38αMAPK pathway can inhibit platelet-derived growth factor-mediated survival activity, resulting in the apoptosis of pericytes and the formation of acellular capillaries [[46]]. PKC-ζ can be detected in endothelial cells and is involved in VEGF-mediated proliferation and hyperpermeability induced by tumor necrosis factor-α (TNF-α) and thrombin (Figure 3) [[47], [48]].

AGEs are the late products of nonenzymatic glycation. Normally, glucose forms a Schiff base through the Maillard reaction; irreversible oxidation and dehydration reactions then convert Amadori products to AGEs [[49]]. Oxidative stress causes the formation of reactive carbonyl compounds, which react with protein, resulting in the production and accumulation of AGEs [[50]]. AGEs can cross-link with structural proteins such as elastin, laminin, and collagen type IV, leading to reduced elasticity and sensitivity to proteolytic digestion [[51]]. In addition, the interaction between AGEs and their cell surface receptor mediates the production of ROS, the activation of NF-κB [[52]], and the expression of VEGF, monocyte chemoattractant protein-1, and plasminogen activator inhibitor-1. This contributes to hyperpermeability and the breakdown of the BRB, ultimately leading to angiogenesis and thrombosis [[53], [54]].

Increased levels of ROS under hyperglycemic conditions inhibit the activity of glyceraldehyde-3-phosphate dehydrogenase, which diverts fructose 6-phosphate from the glycolysis pathway to the hexosamine pathway, thereby activating the hexosamine pathway and producing UDP-N-acetylglucosamine [[55]]. UDP-N-acetylglucosamine is an important substrate in the posttranslational modification of Ser/Thr residues by O-linked β-N-acetylglucosamine. A recent study demonstrated that hyperglycemia promotes the GlcNAcylation of NF-κB, which contributes to the death of retinal gangliocytes [[56]]. Moreover, elevated angiopoietin-2 (Ang-2) levels cause pericyte loss, which is probably related to the increased GlcNAcylation of stimulating protein-3 (Sp-3) through the promotion of Sp-1 binding to the Ang-2 promoter and activation of the transcription of Ang-2 [[57]].

4.2. Nrf2-Keap1 Antioxidant Defense System in DR

Nrf2 is a redox-sensitive factor that can regulate the transcription of antioxidant genes and act as a protective factor in inflammation and ischemia/reperfusion injury [[58], [59]]. Normally, Nrf2 exists in the cytosol and binds to its cytosolic inhibitor Kelch-like ECH-associated protein 1 (Keap1). When oxidative stress occurs, Nrf2 dissociates from Keap1 and translocates to the nucleus, where it binds with the antioxidant-response element (ARE) to regulate the transcription of antioxidant genes (Figure 4) [[7]]. Thus, the Nrf2-Keap1-ARE pathway is considered one of the major ways by which cells are protected from oxidative stress, and it can regulate the expression of a number of protective factors, such as GSH [[7], [60]]. GSH biosynthesis can be regulated by glutamate cysteine ligase (GCL), which has a catalytic subunit (GCLC) and a modifier subunit (GCLM). The transcription of GCLC can be regulated by Nrf2 [[7]]. It has been confirmed that Nrf2 is activated in streptozotocin- (STZ-) induced mouse retinas, and the level of nuclear Nrf2 in diabetic mice is two times higher than in nondiabetic controls. Moreover, in Nrf2-deficient diabetic mice, the levels of ROS, TNF-α, and superoxide increase, and the level of GSH decreases [[7], [61]]. Under high-glucose conditions, there is a decrease of Nrf2 binding at the antioxidant-response element region 4 (ARE4), leading to the decreased expression of its downstream target gene GCLC. Moreover, histone methylation at GCLC-ARE4 can disrupt the binding of Nrf2 with GCLC-ARE4, which is not altered by the termination of high-glucose insult [[62]]. This suggests an important role of the Nrf2-Keap1-GCLC-GSH signaling pathway in the maintenance of retinal redox status in the development of DR.

Epigenetic modifications of Keap1 also play an important role in the development of DR. Keap1 is a crucial factor during Nrf2 signal transduction because it is a substrate adaptor protein for Cullin3/Rbx1 ubiquitin ligase. The Keap1 promoter has an Sp-1 element site, and the binding of Sp-1 with the Keap1 promoter can activate the transcription of Keap1 [[63]]. Hyperglycemia can activate methyltransferase enzyme Set7/9 (SetD7), which facilitates the methylation of lysine 4 on histone 3, and increases the binding of Sp-1 to the Keap1 promoter. Moreover, SetD7-siRNA decreases the binding of Sp-1 to the Keap1 promoter and reduces its expression; thus, the functions of Nrf2 are suppressed. Termination of a high-glucose insult fails to reduce the binding of Sp-1 to Keap1, and the methylation of the promoter persists with the increased expression of Keap1, which implies that it contributes to “metabolic memory” [[64]]. A recent study has shown that NF-κB activity is enhanced by reduction of the deacetylase activity of sirtuin 1 (SIRT1) by high-glucose levels, which leads to downregulation of microRNA-29 (miRNA-29) and inhibition of Keap1/Nrf2 signaling [[65]]. Accumulating evidence supports the protective role of Keap1 in various cancers via regulation by miRNAs such as miRNA-200a and miRNA-141 [[66], [67]], although studies on DR are scarce. This suggests that miRNAs may have therapeutic potential for the clinical treatment of DR.

4.3. Novel Small GTP-Binding Proteins in DR

Apart from the classical mechanisms mentioned above, a few studies have recently demonstrated that small GTP-binding proteins play a crucial role in the pathogenesis of DR; these proteins are one of the biological switches of cellular processes. The family of small GTP-binding proteins is one of the regulators of the signaling cascade triggered by oxidative stress. GTP-binding proteins belong to a superfamily consisting of more than 100 members, which range from 20 to 40 kDa in size; there are five major families: Ras, Rho, Rab, Sar1/Arf, and Ran, which regulate processes such as cellular proliferation, survival, and differentiation [[68]]. These cellular processes cycle between GTP-bound active and GDP-bound inactive states. The Ras and Rho families are regarded as particularly important in the development of DR.

The Ras family is a group of low-molecular weight GTP-binding proteins that are involved in cellular signal transduction; the family includes the three highly homologous proteins H-, K-, and N-Ras [[69]]. It is well known that diabetes increases oxidative stress and that ROS can influence the interactions between H-Ras and its several effector proteins [[70]]. The most important effector protein is Raf-1, a threonine/serine kinase. The Ras/Raf complex can, in turn, induce intracellular oxidative stress [[71]]. It has been demonstrated that the activation of retinal H-Ras in diabetes is mediated by its translocation to the plasma membrane, which can be prevented by simvastatin, an inhibitor that blocks the membrane translocation of H-Ras [[72]]. Evidence suggests that activated H-Ras produces more ROS, probably through activating Nox [[73]]. Moreover, H-Ras activation is thought to be involved in cell migration, proliferation, and differentiation, which leads to neovascularization [[74]]. Previous studies have indicated that the expression levels of H-Ras and its effector protein Raf-1 are increased in the retinas of diabetic animal models, suggesting that H-Ras is involved in retinal capillary apoptosis induced by high-glucose levels; furthermore, inhibitors of retinal H-Ras can prevent the development of DR [[75], [76]]. Several cell regulatory networks cannot be separated from the Ras/Raf/MEK/ERK pathway, which controls a wide range of downstream targets that regulate gene expression and transcription factors (Figure 5) [[69]]. There is also evidence that the Raf/MEK/ERK cascade can be activated by ROS, contributing to cellular dysfunction and/or apoptosis [[77]]. Matrix metalloproteinase-9 (MMP-9), the most complex member of the MMP family, is thought to be involved in vascular permeability and capillary apoptosis in DR owing to its damage to the tight junction complex and activation of caspase-3, respectively [[78]]. Diabetes causes the activation of MMPs in various tissues, and MMP-9 activity is increased in the retinas of patients and animal models with DR [[78], [79]]. However, MMP-9 expression is regulated by H-Ras. In rat liver epithelial cells, H-Ras activation can regulate MMP-9 expression [[80]], and overexpression of H-Ras in human fibroblasts is associated with the upregulation of MMP-9 [[81]]. Furthermore, MMP-9 activation is decreased following treatment with an H-Ras inhibitor [[78]]. This suggests that MMP-9 activation is regulated by H-Ras and that MMP-9 appears to be downstream of H-Ras. However, the exact mechanism remains unclear.

The Rho family and its target protein Rho-kinase (ROCK) are involved in cell migration, adhesion, proliferation, and apoptosis [[68], [82]]. The Rho/ROCK pathway is activated in retinal microvessels during diabetes and affects the expression and function of adhesion molecules, such as ICAM-1, leading to leukocyte adhesion to the microvasculature [[82]]. In endothelial cells, Rho/ROCK signaling activation is also involved in VEGF-induced migration and angiogenesis [[83]]. Moreover, it is well known that transforming growth factor-β (TGF-β) is overexpressed in the vitreous of patients with proliferative vitreoretinal disease and that it contributes to the cicatricial contraction of the membrane. Specifically, TGF-β2, the main isoform in the posterior segment of the eye, activates Rho, leading to myosin light chain phosphorylation in hyalocytes [[84]], which is associated with actin-myosin interaction to form stress fibers and cell contraction [[85], [86]]. This evidence shows that the activation of ROCK is important in the cicatricial contraction of the proliferative membrane in DR patients.

It is well known that the pathogenesis of DR continues after the termination of a high-glucose insult, a phenomenon known as “metabolic memory.” It has been suggested that epigenetic modifications are one of the major factors that contribute to the development of DR. Environmental factors, lifestyle, disease progression, and age influence epigenetic changes; DNA methylation, histone modification, and the effects of miRNAs and sirtuin proteins (SIRTs) are considered the major epigenetic modifications.

4.4.1. DNA Methylation

DNA is not a fixed entity; it can modify its properties to adapt to environmental changes. The methylation of cytosine forms 5-methylated cytosine, which alters the chromatin structure by changing protein-DNA interactions; it is considered to be one of the major epigenetic modifications [[87]]. Normally, CpG islands, which are enriched in the promoter regions, comprise unmethylated cytosines. This guarantees the active expression of genes [[88]]. In experimental studies, hyperglycemic induction is associated with hypermethylation or hypomethylation of the promoter region in some genes that play critical roles in DR development. For example, research shows that a hyperglycemic milieu can alter the methylation status of the MMP-9 promoter, and the regulation of retinal MMP-9 promoter hypomethylation can prevent mitochondrial damage and the development of DR [[89]]. Generally, two classes of enzymes are involved in DNA methylation: DNA methyltransferases (DNMTs) and ten-eleven translocation enzymes. The former can transfer a methyl group from S-adenosyl methionine to cytosine [[90]]; the latter oxidize 5-methylated cytosine to produce 5-hydroxymethyl cytosine, which is easily oxidized to generate 5-formylcytosine and 5-carboxylcytosine [[91]]. DNMT activity is increased and the expression of DNMT1 is elevated in diabetic retinas and their capillary cells [[16]]. In the retinas of rats with STZ-induced diabetes, the promoter region of DNA polymerase gamma is hypermethylated, which is sustained even after 3 months of good glycemic control [[92]]. High levels of glucose also activate ten-eleven translocation enzymes in zebrafish, leading to genome-wide demethylation and aberrant gene expression [[93]]. Therefore, DNA methylation is important in the process of DR and metabolic memory.

4.4.2. Histone Modification

Histone modification is another influential factor in diabetes. Chromatin comprises nucleic acids and histones, which are proteins that can regulate the structure of DNA and the expression of various genes. There are five major histone families: H1/H5, H2A, H2B, H3, and H4. Histones constitute the basic unit, the nucleosome, of chromatin and act as a spool around which the DNA is wound. The N-terminal sequences of histones can be modified by acetylation, phosphorylation, methylation, ubiquitination, or sumoylation [[87]]. Acetylation and methylation are the most common modifications of histones. The acetylation of histones at lysine residues generally occurs with transcriptionally active genes, whereas the methylation of lysine is related to gene activation or repression [[94], [95]]. Two groups of enzymes are involved in acetylation: histone acetyltransferases and histone deacetylases, which can add or remove acetyl groups, respectively. Histone acetyltransferase overactivity results in hyperacetylation, which can facilitate the binding of transcription factors and RNA polymerases to the DNA template [[96]]. There is evidence that, in rats with STZ-induced diabetes, the activity and transcripts of histone deacetylases are increased, and histone acetyltransferase activity is reduced, which leads to the reduced acetylation of histone H3 [[97]]. Another research has shown that modification of histone H3 at lysine 9 (H3K9) at the proximal Cox2 promoter bearing the NF-κB binding site is involved in thioredoxin-interacting protein-mediated inflammation in high-glucose cultured retinal endothelial cells [[98]]. Moreover, transient hyperglycemia can induce upregulation of NF-κB subunit p65, which acts as an inflammatory mediator in diabetes, via active histone H3 lysine 4 monomethylation at the promoter region [[99]]. However, hypomethylation of H3K9 is also observed in diabetes, and this frees up the lysine 9 of H3K9 for acetylation, facilitating the recruitment of NF-κB [[100]]. Furthermore, the promoter and enhancer regions of superoxide dismutase-2 (SOD-2) change in diabetes [[101]], and histone lysine acylation and methylation in monocytes at inflammation- and diabetes-related gene loci can be induced by hyperglycemia [[102], [103]]. Both hyperglycemia and hydrogen peroxide treatment increase the expression of coactivator-associated arginine methyltransferase 1 via histone 3 arginine 17 asymmetric demethylation in RPE cells, suggesting that oxidative stress-mediated cell damage is associated with histone modification [[104], [105]].

4.4.3. MicroRNAs (miRNAs)

MicroRNAs (miRNAs) are small, single-stranded, noncoding RNA molecules. They contain 20–24 nucleotides and regulate genes by binding to the target sequences in the 3′-untranslated regions of messenger RNAs [[106]]. Gene expression can be controlled by miRNA via either degradation or translation repression of messenger RNA, and gene regulation is involved in inflammation, cellular metabolism, angiogenesis, and apoptosis [[107]]. Circulating miRNA is altered in diabetic patients, and the expression of miRNAs can be a predictive factor for the progression of diabetes [[108]]. Recent research has shown that miRNA-200b is upregulated in diabetic mouse retinas. This increases the expression of oxidation resistance-1, which prevents oxidative stress [[109]]; therefore, miRNA-200b has a protective role in DR. Moreover, the expression of miRNA-26b-5p increases after exposure to high levels of glucose [[110]], and miRNA-26b-5p facilitates cardiomyocyte apoptosis by increasing the production of ROS [[111]], suggesting its contribution to endothelial apoptosis in DR. Other experiments have provided evidence that oxidative stress regulates SIRT1, which has significant antioxidative and anti-inflammatory effects through miRNA; several miRNAs such as miRNA-217, miRNA-181, miRNA-138, and miRNA-199 downregulate SIRT1 in various cells and tissue types [[112]]. These studies suggest that miRNAs play an important role in regulating gene expression in DR.

4.4.4. SIRTs

SIRTs are homologs of silent information regulator 2 in higher eukaryotic organisms. They participate in the epigenetic modifications of histone and nonhistone protein deacetylation, which contribute to the mechanisms of vascular dysfunction related to aging, cardiovascular disease, diabetes, and other metabolic diseases [[113], [114]]. Mammals have seven SIRTs (SIRT1–7) that all depend on NAD+ cofactor. SIRT1 and SIRT3 are closely correlated with diabetes and its complications. This is particularly true of SIRT1 [[115], [116]], which regulates oxidative stress response and RPE cell apoptosis via the deacetylation of cytoplasmic p53 [[117]]. A recent study has shown that SIRT1 activity decreases in diabetic retinas [[118]]. Moreover, SIRT1 overexpression protects pancreatic β-cells by inhibiting the NF-κB pathway through deacetylation of p65 [[119]]. In cultured mammalian cells, SIRT1 is activated in response to energy stress and increased oxidative stress. Evidence also shows that SIRT1 is closely related to redox modulation because its activity depends on intracellular NAD+[[120]]. Furthermore, exendin-4 reduces the number of retinal cells and the production of ROS by upregulating the expression of SIRT1 and SIRT3 in the retinas of early-stage diabetic rats [[121]].

5. Antioxidant Treatments for DR

In the previous sections, we have demonstrated that oxidative stress plays a crucial role in the development of DR. Therefore, it is postulated that antioxidants inhibit abnormal metabolism and slow DR progression by inhibiting the production of ROS, neutralizing free radicals, or augmenting the antioxidant defense system. Therefore, these factors are targets in the treatment of DR.

5.1. Vitamins

Vitamins C and E can protect against the development of DR by scavenging free radicals, reducing the production of ROS, and preventing lipid peroxidation [[122], [123]]. Therefore, supplementation with vitamins C and E is thought to be helpful in the treatment of DR. Vitamin C can ameliorate endothelial dysfunction in diabetes and decrease leukocyte adhesion in retinal vessels in diabetic rats [[123]]. Vitamin E inhibits the PKC pathway in diabetes; the PKC pathway induces a decrease of retinal blood flow, resulting in retinopathy [[124]]. Recently, vitamin D has attracted interest as being important in the development of DR. Research shows that vitamin D deficiency is very common in type II diabetes, and there is an increased risk of retinopathy in diabetic patients with severe vitamin D deficiency [[125]]. Further research has demonstrated that vitamin D can inhibit inflammation as well as the expression of VEGF and TGF-β1 in retinal tissues, thereby protecting the retina from neovascularization and cell proliferation [[126]].

5.2. Green Tea

Green tea, which is rich in polyphenols, is thought to have potent antioxidative, anti-inflammatory, and anticarcinogenic properties [[127], [128]]. Green tea supplementation in diabetic rats increases the level of GSH and the activities of SOD and catalase, reduces the expression of VEGF and TNF-α, and protects retinal vessels from angiogenesis and retinal endothelial cells from apoptosis [[128]]. These results indicate the possible mechanisms of the beneficial effects of green tea in the treatment of DR.

5.3. α-Lipoic Acid (ALA)

ALA is a powerful antioxidant that is necessary for the function of several enzymes involved in oxidative metabolism [[129]]. It can reduce the amounts of oxidized forms of other antioxidants such as GSH and vitamins C and E and can modulate several of the signal transduction pathways involved in vascular damage, such as the NF-κB pathway [[130]]. It has been shown that ALA supplementation in diabetic rats over 30 weeks can prevent damage to retinal vessels, including pericyte dropout, and ALA can also inhibit the expression of Ang-2, VEGF, and AGE receptors, which are related to pericyte dropout, neovascularization, and the activation of NF-κB [[131]].

5.4. Benfotiamine

Benfotiamine, a lipophilic analogue of thiamine monophosphate, can protect vascular cells from the metabolic damage induced by hyperglycemia. In diabetic animals, benfotiamine has been shown to inhibit three major ROS production pathways (the hexosamine, PKC, and AGE pathways) and can reduce the activation of NF-κB by exciting transketolase [[132]]. There is also evidence that benfotiamine can protect retinal pericytes from apoptosis via normalizing the Bcl-2/Bax ratio [[133]]. Therefore, benfotiamine might provide an alternative therapy for DR.

5.5. Poly (ADP-Ribose) Polymerase (PARP) Inhibitors

PARP is one of the downstream oxidant injury mediators. It is involved in retinal cell apoptosis and the regulation of NF-κB activation [[134]]. PARP can increase the expression of vasoactive factors and the production of extracellular matrix proteins in diabetic animals, and the genetic and chemical blockade of PARP can normalize these changes [[135]]. Administration of PJ-34, a potent PARP inhibitor, decreases the activation of NF-κB and the transcription of NF-κB-dependent genes such as ICAM-1 in diabetic rats [[136]]. The evidence supports the value of PARP as a therapeutic target for delaying the development of DR.

5.6. SOD

SOD, the first line of physiological defense against oxidative stress, also contributes to the development of DR. The overexpression of manganese SOD can decrease the hyperglycemia-induced expression of VEGF mRNA and protein in endothelial cells, and the expression of VEGF and fibronectin in STZ-induced DR models can also be reduced [[137]]. Moreover, overexpression of mitochondrial SOD in hemizygous transgenic mice can prevent diabetic retinal oxidative stress and mitochondrial dysfunction [[138]]. Tempol, a SOD mimetic, can scavenge O2•−, reduce the amount of NO end-products, and increase GSH biosynthesis, thereby normalizing the oxidative/nitrosative status of DR. Furthermore, decreased copper-zinc SOD expression/activity in treated diabetic rats has been observed [[139]].

5.7. Other Antioxidants

Other antioxidants also have beneficial effects on the development of DR. For example, zinc, an important nutrient, is involved in oxidative stress, apoptosis, and aging. Zinc can prevent the increase of lipid peroxidation induced by diabetes in the retina and can reduce GSH levels in diabetic rats [[140]]. Moreover, Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) and N-acetyl-L-cysteine prevent glycated albumin-induced cell death in pericytes [[141]]. Nicanartine, an antioxidant with cholesterol-lowering properties, can partially inhibit pericyte loss in diabetic rats [[142]]. Curcumin, a traditional herb extract, demonstrates significant antioxidant activity by downregulating hypoxia-inducible factor 1 and preventing angiogenesis induced by hypoxia [[143]]. Curcumin supplementation can inhibit the expression of VEGF in DR rats [[144]]. Furthermore, β-carotene, taurine, lutein, caffeic acid phenethyl ester, cannabidiol, and 8-hydroxy-N,N-dipropyl-2-aminotetralin also have some antioxidant properties [[145]].

In the previous sections, we have described the role of Nrf2-signaling pathways in DR and inflammation [[58]]. Sulforaphane, an Nrf2 inducer, can protect the RPE against oxidative injury [[146]]. Hemin is another potential treatment. In diabetic rats, the levels of heme oxygenase-1, SOD-1, and Bcl-2 increase, whereas the expressions levels of hypoxia-inducible factor 1 and VEGF decrease following treatment with hemin. The activation of Nrf2 nuclear translocation contributes to these results [[147]]. Mycophenolate mofetil (MMF) is also considered an effective drug. Evidence shows that MMF facilitates Nrf2 nuclear translocation and conserves antioxidant enzymes in diabetic nephropathy [[148]]. Moreover, zinc, oltipraz, and dimethyl fumarate have different effects in diabetes via the Nrf2 pathway [[149][151]].

As mentioned above, DNA methylation, histone modification, and miRNAs are three major epigenetic factors that are potential targets for the treatment of diabetes. DNA methylation can be repressed by nucleoside analogues because they inhibit DNMTs. Moreover, DNMT inhibitors, including 5-azacytidine (azacitidine; Vidaza) and 5-aza-20-deoxycytidine (decitabine; Dacogen) for myeloid cancers and cutaneous T cell lymphoma, have been recommended by the US Food and Drug Administration [[87]]. The regulation of histone modification also has a large effect. For instance, epigallocatechin-3-gallate, a strong histone acetyltransferases inhibitor, can block the activation of p65 acetylation-dependent NF-κB [[152]]. Curcumin, genistein, and resveratrol also have an influence on histone modification [[153]]. Resveratrol has a protective role against inflammation because it controls the expression of several miRNAs such as miRNA-21, miRNA-155, and miRNA-663 [[154]]. For miRNA, double-stranded miRNA mimics and anti-RNA antisense oligodeoxyribonucleotides are major molecules that have been investigated for targeting specific miRNAs [[9]]. Metformin, which is involved in the SIRT1/LKB1/AMPK pathway, suppresses hyperglycemia stress “memory” in diabetic rats [[155]]. Another research has shown that cocoa enriched with polyphenol improves the retinal SIRT1 pathway and protects the retina from diabetic insult [[156]]. Moreover, resveratrol and fenofibrate also have a protective effect on DR via SIRT1-related pathways [[118], [157]].

6. Conclusions

DR is a leading cause of blindness in working-age adults, and oxidative stress plays an important role in its development. In this review, we have demonstrated that hyperglycemia induces a series of metabolic abnormalities in the retina by producing ROS and subsequently initiating oxidative stress. This can in turn activate these abnormal metabolic pathways to produce more ROS, thereby creating a vicious cycle. Generally, oxidative stress causes retinal damage by inducing endothelial cell dysfunction, pericyte apoptosis, and angiogenesis. The polyol, AGE, PKC, hexosamine, and Nrf2-related pathways, GTP-binding proteins, and epigenetic modifications are all involved in the pathological process of DR. Therefore, inhibitors of these pathways may protect the retina from damage induced by high levels of glucose. Antioxidants are beneficial for DR because they reduce ROS production, neutralize free peroxynitrite, or augment the antioxidant defense system. Furthermore, herbal extracts have received increasing attention for treating DR in recent years, although the mechanisms underlying their mode of action require verification. At present, most of the evidence has been provided by animal experiments, and the clinical therapeutic effects are still not very clear. Thus, more work is needed in the future.

Acknowledgements

Acknowledgments

This study was supported by the National Natural Science Foundation of China (81400279, to Yonggang Wang).

References

  1. N. H. ChoQ&A: five questions on the 2015 IDF diabetes atlasDiabetes Research and Clinical Practice201611515715927242128
  2. S. ResnikoffD. PascoliniD. Etya'aleGlobal data on visual impairment in the year 2002Bulletin of the World Health Organization200482118448512-s2.0-964428308915640920
  3. Y. ZhengM. HeN. CongdonThe worldwide epidemic of diabetic retinopathyIndian Journal of Ophthalmology201260542843110.4103/0301-4738.1005422-s2.0-8486635805422944754
  4. R. N. FrankDiabetic retinopathyThe New England Journal of Medicine20043501485810.1056/nejmra0216782-s2.0-034804887414702427
  5. F. GiaccoM. BrownleeOxidative stress and diabetic complicationsCirculation Research201010791058107010.1161/CIRCRESAHA.110.2235452-s2.0-7834929756521030723
  6. R. A. KowluruP.-S. ChanOxidative stress and diabetic retinopathyExperimental Diabesity Research20072007124360310.1155/2007/436032-s2.0-34247394257
  7. Q. ZhongM. MishraR. A. KowluruTranscription factor Nrf2-mediated antioxidant defense system in the development of diabetic retinopathyInvestigative Ophthalmology & Visual Science20135463941394810.1167/iovs.13-115982-s2.0-8487880432723633659
  8. G. MohammadR. A. KowluruThe role of Raf-1 kinase in diabetic retinopathyExpert Opinion on Therapeutic Targets201115435736410.1517/14728222.2011.5536042-s2.0-7995264246621254925
  9. R. A. KowluruJ. M. SantosM. MishraEpigenetic modifications and diabetic retinopathyBioMed Research International20132013963528410.1155/2013/6352842-s2.0-84888589850
  10. R. V. IntineM. P. Sarras Jr.Metabolic memory and chronic diabetes complications: potential role for epigenetic mechanismsCurrent Diabetes Reports201212555155910.1007/s11892-012-0302-72-s2.0-8487084724122760445
  11. S. SharmaS. SaxenaK. SrivastavNitric oxide and oxidative stress is associated with severity of diabetic retinopathy and retinal structural alterationsClinical & Experimental Ophthalmology201543542943610.1111/ceo.125062-s2.0-8493702289025675974
  12. T.-R. GoldenS. MelovMitochondrial DNA mutations, oxidative stress, and agingMechanisms of Ageing and Development2001122141577158910.1016/S0047-6374(01)00288-32-s2.0-003597571311511398
  13. J.-M. LiA. M. ShahROS generation by nonphagocytic NADPH oxidase: potential relevance in diabetic nephropathyJournal of the American Society of Nephrology2003148, supplement 3S221S22610.1097/01.asn.0000077406.67663.e72-s2.0-004186537212874435
  14. Y. DuC. M. MillerT. S. KernHyperglycemia increases mitochondrial superoxide in retina and retinal cellsFree Radical Biology and Medicine200335111491149910.1016/j.freeradbiomed.2003.08.0182-s2.0-034401201514642397
  15. S. A. Madsen-BouterseG. MohammadM. KanwarR. A. KowluruRole of mitochondrial DNA damage in the development of diabetic retinopathy, and the metabolic memory phenomenon associated with its progressionAntioxidants & Redox Signaling201013679780510.1089/ars.2009.29322-s2.0-7795490905420088705
  16. M. MishraR. A. KowluruEpigenetic modification of mitochondrial DNA in the development of diabetic retinopathyInvestigative Ophthalmology & Visual Science20155695133514210.1167/iovs.15-169372-s2.0-8493983894426241401
  17. C. SzabóH. IschiropoulosR. RadiPeroxynitrite: biochemistry, pathophysiology and development of therapeuticsNature Reviews Drug Discovery20076866268010.1038/nrd22222-s2.0-3454767349717667957
  18. I. M. GoldsteinP. OstwaldS. RothNitric oxide: a review of its role in retinal function and diseaseVision Research199636182979299410.1016/0042-6989(96)00017-x2-s2.0-00302499448917798
  19. J. M. SantosS. TewariR. A. KowluruA compensatory mechanism protects retinal mitochondria from initial insult in diabetic retinopathyFree Radical Biology & Medicine20125391729173710.1016/j.freeradbiomed.2012.08.5882-s2.0-8486737418822982046
  20. T. AgoJ. KurodaM. KamouchiJ. SadoshimaT. KitazonoPathophysiological roles of NADPH oxidase/Nox family proteins in the vascular system—review and perspectiveCirculation Journal20117581791180010.1253/circj.cj-11-03882-s2.0-7996079299321673456
  21. M. RojasW. ZhangZ. XuRequirement of NOX2 expression in both retina and bone marrow for diabetes-induced retinal vascular injuryPLoS ONE2013812e8435710.1371/journal.pone.00843572-s2.0-84892910910
  22. F. ChenL.-H. QianB. DengZ.-M. LiuY. ZhaoY.-Y. LeResveratrol protects vascular endothelial cells from high glucose-induced apoptosis through inhibition of NADPH oxidase activation-driven oxidative stressCNS Neuroscience & Therapeutics201319967568110.1111/cns.121312-s2.0-8488291678323731528
  23. H. DingM. AljofanC. R. TriggleOxidative stress and increased eNOS and NADPH oxidase expression in mouse microvessel endothelial cellsJournal of Cellular Physiology2007212368268910.1002/jcp.210632-s2.0-3454794642317443690
  24. P. WeidigD. McMasterU. BayraktutanHigh glucose mediates pro-oxidant and antioxidant enzyme activities in coronary endothelial cellsDiabetes, Obesity & Metabolism20046643244110.1111/j.1462-8902.2004.00364.x2-s2.0-6944237098
  25. J. Cunha-VazR. BernardesNonproliferative retinopathy in diabetes type 2. Initial stages and characterization of phenotypesProgress in Retinal and Eye Research200524335537710.1016/j.preteyeres.2004.07.0042-s2.0-1374424912015708833
  26. S. RoyT. SatoG. ParyaniR. KaoDownregulation of fibronectin overexpression reduces basement membrane thickening and vascular lesions in retinas of galactose-fed ratsDiabetes20035251229123410.2337/diabetes.52.5.12292-s2.0-003740731412716757
  27. J. KimC.-S. KimY. M. LeeK. JoS. D. ShinJ. S. KimMethylglyoxal induces hyperpermeability of the blood-retinal barrier via the loss of tight junction proteins and the activation of matrix metalloproteinasesGraefe's Archive for Clinical and Experimental Ophthalmology2012250569169710.1007/s00417-011-1912-52-s2.0-84862903126
  28. V. E. KaganA. A. ShvedovaK. N. NovikovY. P. KozlovLight-induced free radical oxidation of membrane lipids in photoreceptors of frog retinaBiochimica et Biophysica Acta—Biomembranes19733301767910.1016/0005-2736(73)90285-x2-s2.0-0015856911
  29. M. E. HartnettR. D. StrattonR. W. BrowneB. A. RosnerR. J. LanhamD. ArmstrongSerum markers of oxidative stress and severity of diabetic retinopathyDiabetes Care200023223424010.2337/diacare.23.2.2342-s2.0-003397929110868837
  30. R. A. KowluruP. KoppoluDiabetes-induced activation of caspase-3 in retina: effect of antioxidant therapyFree Radical Research200236999399910.1080/10715760210000065722-s2.0-003602331412448825
  31. R. A. KowluruP. KoppoluS. ChakrabartiS. ChenDiabetes-induced activation of nuclear transcriptional factor in the retina, and its inhibition by antioxidantsFree Radical Research200337111169118010.1080/107157603100016041892-s2.0-014218004714703729
  32. Y. DuA. VeenstraK. PalczewskiT. S. KernPhotoreceptor cells are major contributors to diabetes-induced oxidative stress and local inflammation in the retinaProceedings of the National Academy of Sciences of the United States of America201311041165861659110.1073/pnas.13145751102-s2.0-8488535761724067647
  33. B. A. BerkowitzE. M. GradyN. KhetarpalA. PatelR. RobertsOxidative stress and light-evoked responses of the posterior segment in a mouse model of diabetic retinopathyInvestigative Ophthalmology & Visual Science201556160661510.1167/iovs.14-156872-s2.0-8492193508225574049
  34. G. B. ArdenThe absence of diabetic retinopathy in patients with retinitis pigmentosa: implications for pathophysiology and possible treatmentThe British Journal of Ophthalmology200185336637010.1136/bjo.85.3.3662-s2.0-003508699211222350
  35. G. B. ArdenJ. E. WolfY. TsangDoes dark adaptation exacerbate diabetic retinopathy? Evidence and a linking hypothesisVision Research199838111723172910.1016/S0042-6989(98)00004-22-s2.0-00321023369747505
  36. T. E. de GooyerK. A. StevensonP. HumphriesD. A. C. SimpsonT. A. GardinerA. W. StittRetinopathy is reduced during experimental diabetes in a mouse model of outer retinal degenerationInvestigative Ophthalmology & Visual Science200647125561556810.1167/iovs.06-06472-s2.0-3424821512717122149
  37. P. PacherI. G. ObrosovaJ. G. MableyC. SzabóRole of nitrosative stress and peroxynitrite in the pathogenesis of diabetic complications. Emerging new therapeutical strategiesCurrent Medicinal Chemistry200512326727510.2174/09298670533632072-s2.0-1454429796115723618
  38. E. C. LealA. ManivannanK.-I. HosoyaInducible nitric oxide synthase isoform is a key mediator of leukostasis and blood-retinal barrier breakdown in diabetic retinopathyInvestigative Ophthalmology & Visual Science200748115257526510.1167/iovs.07-01122-s2.0-3844910861617962481
  39. Z. YuanW. FengJ. HongQ. ZhengJ. ShuaiY. Gep38MAPK and ERK promote nitric oxide production in cultured human retinal pigmented epithelial cells induced by high concentration glucoseNitric Oxide: Biology and Chemistry200920191510.1016/j.niox.2008.09.0012-s2.0-5554914215218854222
  40. K. H. GabbayThe sorbitol pathway and the complications of diabetesThe New England Journal of Medicine19732881683183610.1056/nejm1973041928816092-s2.0-00159297394266466
  41. D. G. CoganJ. H. KinoshitaP. F. KadorNIH conference. Aldose reductase and complications of diabetesAnnals of Internal Medicine19841011829110.7326/0003-4819-101-1-822-s2.0-00211958476203454
  42. A. B. El-RemessyG. Abou-MohamedR. W. CaldwellR. B. CaldwellHigh glucose-induced tyrosine nitration in endothelial cells: role of eNOS uncoupling and aldose reductase activationInvestigative Ophthalmology & Visual Science20034473135314310.1167/iovs.02-10222-s2.0-003756252312824263
  43. M. ClarkeP. M. DodsonPKC inhibition and diabetic microvascular complicationsBest Practice & Research Clinical Endocrinology & Metabolism200721457358610.1016/j.beem.2007.09.0072-s2.0-3654900261018054736
  44. K. SuzumaN. TakaharaI. SuzumaCharacterization of protein kinase C β isoform's action on retinoblastoma protein phosphorylation, vascular endothelial growth factor-induced endothelial cell proliferation, and retinal neovascularizationProceedings of the National Academy of Sciences of the United States of America200299272172610.1073/pnas.0226444992-s2.0-003715415611805327
  45. J. ParkH. KimS. Y. ParkTonicity-responsive enhancer binding protein regulates the expression of aldose reductase and protein kinase C δ in a mouse model of diabetic retinopathyExperimental Eye Research2014122131910.1016/j.exer.2014.03.0012-s2.0-8489695624124631337
  46. P. GeraldesJ. Hiraoka-YamamotoM. MatsumotoActivation of PKC-delta and SHP-1 by hyperglycemia causes vascular cell apoptosis and diabetic retinopathyNature Medicine200915111298130610.1038/nm.20522-s2.0-70449108112
  47. C. A. AveleiraC. M. LinS. F. AbcouwerA. F. AmbrosioD. A. AntonettiTNF-alpha signals through PKCzeta/NF-kappaB to alter the tight junction complex and increase retinal endothelial cell permeabilityDiabetes201059112872288220693346
  48. R. D. MinshallE. E. VandenbrouckeM. HolinstatRole of protein kinase Czeta in thrombin-induced RhoA activation and inter-endothelial gap formation of human dermal microvessel endothelial cell monolayersMicrovascular Research201080224024920417648
  49. E. D. SchleicherA. BierhausH.-U. HäringP. P. NawrothR. LehmannChemistry and pathobiology of advanced glycation end productsContributions to Nephrology2001131192-s2.0-0035526694
  50. A. W. StittAGEs and diabetic retinopathyInvestigative Ophthalmology & Visual Science201051104867487410.1167/iovs.10-58812-s2.0-7795812484220876889
  51. D. A. KassE. P. ShapiroM. KawaguchiImproved arterial compliance by a novel advanced glycation end-product crosslink breakerCirculation2001104131464147010.1161/hc3801.0978062-s2.0-003594950211571237
  52. A. GoldinJ. A. BeckmanA. M. SchmidtM. A. CreagerAdvanced glycation end products: sparking the development of diabetic vascular injuryCirculation2006114659760510.1161/circulationaha.106.6218542-s2.0-3374692033716894049
  53. S. YoshidaY. KuboY. KobayashiIncreased vitreous concentrations of MCP-1 and IL-6 after vitrectomy in patients with proliferative diabetic retinopathy: possible association with postoperative macular oedemaThe British Journal of Ophthalmology201599796096610.1136/bjophthalmol-2014-3063662-s2.0-8493773398625631486
  54. S.-I. YamagishiK. NakamuraT. MatsuiPigment epithelium-derived factor inhibits advanced glycation end product-induced retinal vascular hyperpermeability by blocking reactive oxygen species-mediated vascular endothelial growth factor expressionThe Journal of Biological Chemistry200628129202132022010.1074/jbc.m6021102002-s2.0-3374601993916707486
  55. X. DuT. MatsumuraD. EdelsteinInhibition of GAPDH activity by poly(ADP-ribose) polymerase activates three major pathways of hyperglycemic damage in endothelial cellsThe Journal of Clinical Investigation200311271049105710.1172/jci2003181272-s2.0-024228569914523042
  56. S. J. KimW. S. YooM. ChoiI. ChungJ. M. YooW. S. ChoiIncreased O-GlcNAcylation of NF-kappaB enhances retinal ganglion cell death in Streptozotocin-induced diabetic retinopathyCurrent Eye Research201641224925725835259
  57. D. YaoT. TaguchiT. MatsumuraHigh glucose increases angiopoietin-2 transcription in microvascular endothelial cells through methylglyoxal modification of mSin3AThe Journal of Biological Chemistry200728242310383104510.1074/jbc.m7047032002-s2.0-3564902677517670746
  58. Q. MaL. BattelliA. F. HubbsMultiorgan autoimmune inflammation, enhanced lymphoproliferation, and impaired homeostasis of reactive oxygen species in mice lacking the antioxidant-activated transcription factor Nrf2The American Journal of Pathology200616861960197410.2353/ajpath.2006.0511132-s2.0-3374472937316723711
  59. Y. WeiJ. GongT. YoshidaNrf2 has a protective role against neuronal and capillary degeneration in retinal ischemia-reperfusion injuryFree Radical Biology & Medicine201151121622410.1016/j.freeradbiomed.2011.04.0262-s2.0-7995795449221545836
  60. T. W. KenslerN. WakabayashiS. BiswalCell survival responses to environmental stresses via the Keap1-Nrf2-ARE pathwayAnnual Review of Pharmacology and Toxicology2007478911610.1146/annurev.pharmtox.46.120604.1410462-s2.0-33847050801
  61. Z. XuY. WeiJ. GongNRF2 plays a protective role in diabetic retinopathy in miceDiabetologia201457120421310.1007/s00125-013-3093-82-s2.0-8489098202724186494
  62. M. MishraQ. ZhongR. A. KowluruEpigenetic modifications of Nrf2-mediated glutamate-cysteine ligase: implications for the development of diabetic retinopathy and the metabolic memory phenomenon associated with its continued progressionFree Radical Biology & Medicine20147512913910.1016/j.freeradbiomed.2014.07.0012-s2.0-8490633149925016074
  63. D. GuoB. WuJ. YanX. LiH. SunD. ZhouA possible gene silencing mechanism: hypermethylation of the Keap1 promoter abrogates binding of the transcription factor Sp1 in lung cancer cellsBiochemical and Biophysical Research Communications20124281808510.1016/j.bbrc.2012.10.0102-s2.0-8486836300223047008
  64. M. MishraQ. ZhongR. A. KowluruEpigenetic modifications of Keap1 regulate its interaction with the protective factor Nrf2 in the development of diabetic retinopathyInvestigative Ophthalmology & Visual Science201455117256726510.1167/iovs.14-151932-s2.0-8492188942825301875
  65. L. ZhouD.-Y. XuW.-G. ShaHigh glucose induces renal tubular epithelial injury via Sirt1/NF-kappaB/microR-29/Keap1 signal pathwayJournal of Translational Medicine2015131, article 35210.1186/s12967-015-0710-y2-s2.0-84946558501
  66. G. EadesM. YangY. YaoY. ZhangQ. ZhoumiR-200a regulates Nrf2 activation by targeting Keap1 mRNA in breast cancer cellsThe Journal of Biological Chemistry201128647407254073310.1074/jbc.m111.2754952-s2.0-8175517145121926171
  67. X. ZhouJ. SuL. ZhuG. ZhangHelicobacter pylori modulates cisplatin sensitivity in gastric cancer by down-regulating miR-141 expressionHelicobacter201419317418110.1111/hel.121202-s2.0-8489949224224628843
  68. Y. TakaiT. SasakiT. MatozakiSmall GTP-binding proteinsPhysiological Reviews20018111532082-s2.0-003513592911152757
  69. S. SchubbertG. BollagK. ShannonDeregulated Ras signaling in developmental disorders: new tricks for an old dogCurrent Opinion in Genetics & Development2007171152210.1016/j.gde.2006.12.0042-s2.0-3384620754617208427
  70. T.-H. ChengN.-L. ShihS.-Y. ChenReactive oxygen species mediate cyclic strain-induced endothelin-1 gene expression via Ras/Raf/extracellular signal-regulated kinase pathway in endothelial cellsJournal of Molecular and Cellular Cardiology200133101805181410.1006/jmcc.2001.14442-s2.0-003477124111603923
  71. G. CudaR. PaternòR. CeravoloProtection of human endothelial cells from oxidative stress: role of Ras-ERK1/2 signalingCirculation2002105896897410.1161/hc0802.1043242-s2.0-003717693711864927
  72. R. A. KowluruM. KanwarTranslocation of H-Ras and its implications in the development of diabetic retinopathyBiochemical and Biophysical Research Communications2009387346146610.1016/j.bbrc.2009.07.0382-s2.0-6824911656819607814
  73. K. IraniY. XiaJ. L. ZweierMitogenic signaling mediated by oxidants in Ras-transformed fibroblastsScience199727553061649165210.1126/science.275.5306.16492-s2.0-00309806419054359
  74. K. N. MeadowsP. BryantP. A. VincentK. M. PumigliaActivated Ras induces a proangiogenic phenotype in primary endothelial cellsOncogene200423119220010.1038/sj.onc.12069212-s2.0-094230117114712224
  75. R. A. KowluruA. KowluruS. ChakrabartiZ. KhanPotential contributory role of H-Ras, a small G-protein, in the development of retinopathy in diabetic ratsDiabetes200453377578310.2337/diabetes.53.3.7752-s2.0-144227654514988264
  76. M. KanwarR. A. KowluruDiabetes regulates small molecular weight G-protein, H-Ras, in the microvasculature of the retina: implication in the development of retinopathyMicrovascular Research200876318919310.1016/j.mvr.2008.04.0022-s2.0-5364909033918514235
  77. V. KowluruR. A. KowluruIncreased oxidative stress in diabetes regulates activation of a small molecular weight G-protein, H-Ras, in the retinaMolecular Vision2007136026102-s2.0-3424740305117515880
  78. R. A. KowluruRole of matrix metalloproteinase-9 in the development of diabetic retinopathy and its regulation by H-RasInvestigative Ophthalmology and Visual Science20105184320432610.1167/iovs.09-48512-s2.0-7795586542720220057
  79. A. DasA. McLamoreW. SongP. G. McGuireRetinal neovascularization is suppressed with a matrix metalloproteinase inhibitorArchives of Ophthalmology1999117449850310.1001/archopht.117.4.4982-s2.0-003295565910206578
  80. K. W. LeeM.-S. KimN. J. KangH-Ras selectively up-regulates MMP-9 and COX-2 through activation of ERK1/2 and NF-κB: an implication for invasive phenotype in rat liver epithelial cellsInternational Journal of Cancer200611981767177510.1002/ijc.220562-s2.0-3374848950816721810
  81. T. A. GiambernardiG. M. GrantG. P. TaylorOverview of matrix metalloproteinase expression in cultured human cellsMatrix Biology199816848349610.1016/S0945-053X(98)90019-12-s2.0-00319150489550265
  82. R. AritaY. HataS. NakaoRho kinase inhibition by fasudil ameliorates diabetes-induced microvascular damageDiabetes200958121522610.2337/db08-07622-s2.0-6294915022918840783
  83. M. V. HoangM. C. WhelanD. R. SengerRho activity critically and selectively regulates endothelial cell organization during angiogenesisProceedings of the National Academy of Sciences of the United States of America200410171874187910.1073/pnas.03085251002-s2.0-124229687114769914
  84. K. HirayamaY. HataY. NodaThe involvement of the Rho-kinase pathway and its regulation in cytokine-induced collagen gel contraction by hyalocytesInvestigative Ophthalmology & Visual Science200445113896390310.1167/iovs.03-13302-s2.0-694425346915505034
  85. K. E. KammJ. T. StullThe function of myosin and myosin light chain kinase phosphorylation in smooth muscleAnnual Review of Pharmacology and Toxicology19852559362010.1146/annurev.pa.25.040185.0031132-s2.0-0021894617
  86. Y. FukataM. AmanoK. KaibuchiRho-Rho-kinase pathway in smooth muscle contraction and cytoskeletal reorganization of non-muscle cellsTrends in Pharmacological Sciences2001221323910.1016/s0165-6147(00)01596-011165670
  87. R. A. KowluruA. KowluruM. MishraB. KumarOxidative stress and epigenetic modifications in the pathogenesis of diabetic retinopathyProgress in Retinal and Eye Research201548406110.1016/j.preteyeres.2015.05.0012-s2.0-8493926008325975734
  88. A. M. DeatonA. BirdCpG islands and the regulation of transcriptionGenes & Development201125101010102210.1101/gad.20375112-s2.0-7995633096421576262
  89. R. A. KowluruY. ShanM. MishraDynamic DNA methylation of matrix metalloproteinase-9 in the development of diabetic retinopathyLaboratory Investigation201696101040104910.1038/labinvest.2016.7827454437
  90. S. MajumdarE. BucklesJ. EstradaS. KoochekpourAberrant DNA methylation and prostate cancerCurrent Genomics201112748650510.2174/1389202117979040612-s2.0-8005511846122547956
  91. P. ZhangB. HuangX. XuW. C. SessaTen-eleven translocation (Tet) and thymine DNA glycosylase (TDG), components of the demethylation pathway, are direct targets of miRNA-29aBiochemical and Biophysical Research Communications2013437336837310.1016/j.bbrc.2013.06.0822-s2.0-8488094768823820384
  92. S. TewariQ. ZhongJ. M. SantosR. A. KowluruMitochondria DNA replication and DNA methylation in the metabolic memory associated with continued progression of diabetic retinopathyInvestigative Ophthalmology & Visual Science20125384881488810.1167/iovs.12-97322-s2.0-8486732043822743328
  93. N. DhliwayoM. P. Sarras Jr.E. LuczkowskiS. M. MasonR. V. IntineParp inhibition prevents ten-eleven translocase enzyme activation and hyperglycemia-induced DNA demethylationDiabetes20146393069307610.2337/db13-19162-s2.0-8490666365424722243
  94. T. KouzaridesChromatin modifications and their functionCell2007128469370510.1016/j.cell.2007.02.0052-s2.0-3384707684917320507
  95. C. MartinY. ZhangThe diverse functions of histone lysine methylationNature Reviews Molecular Cell Biology200561183884910.1038/nrm17612-s2.0-2764458967516261189
  96. M. WegnerD. NeddermannM. Piorunska-StolzmannP. P. JagodzinskiRole of epigenetic mechanisms in the development of chronic complications of diabetesDiabetes Research and Clinical Practice2014105216417510.1016/j.diabres.2014.03.0192-s2.0-8490604344324814876
  97. Q. ZhongR. A. KowluruRole of histone acetylation in the development of diabetic retinopathy and the metabolic memory phenomenonJournal of Cellular Biochemistry201011061306131310.1002/jcb.226442-s2.0-7795549870020564224
  98. L. PerroneT. S. DeviK.-I. HosoyaT. TerasakiL. P. SinghThioredoxin interacting protein (TXNIP) induces inflammation through chromatin modification in retinal capillary endothelial cells under diabetic conditionsJournal of Cellular Physiology2009221126227210.1002/jcp.218522-s2.0-6924922635319562690
  99. D. BrasacchioJ. OkabeC. TikellisHyperglycemia induces a dynamic cooperativity of histone methylase and demethylase enzymes associated with gene-activating epigenetic marks that coexist on the lysine tailDiabetes20095851229123610.2337/db08-16662-s2.0-6554917030319208907
  100. Q. ZhongR. A. KowluruRegulation of matrix metalloproteinase-9 by epigenetic modifications and the development of diabetic retinopathyDiabetes20136272559256810.2337/db12-11412-s2.0-8487877433423423566
  101. Q. ZhongR. A. KowluruEpigenetic modification of Sod2 in the development of diabetic retinopathy and in the metabolic memory: role of histone methylationInvestigative Ophthalmology & Visual Science201354124425010.1167/iovs.12-108542-s2.0-8487453420823221071
  102. F. MiaoX. WuL. ZhangY.-C. YuanA. D. RiggsR. NatarajanGenome-wide analysis of histone lysine methylation variations caused by diabetic conditions in human monocytesThe Journal of Biological Chemistry200728218138541386310.1074/jbc.m6094462002-s2.0-3425030310417339327
  103. J.-M. YunI. JialalS. DevarajEpigenetic regulation of high glucose-induced proinflammatory cytokine production in monocytes by curcuminThe Journal of Nutritional Biochemistry201122545045810.1016/j.jnutbio.2010.03.0142-s2.0-7995460283320655188
  104. D.-I. KimM.-J. ParkS.-K. LimHigh-glucose-induced CARM1 expression regulates apoptosis of human retinal pigment epithelial cells via histone 3 arginine 17 dimethylation: role in diabetic retinopathyArchives of Biochemistry and Biophysics2014560364310.1016/j.abb.2014.07.0212-s2.0-8490735721725072916
  105. D.-I. KimM.-J. ParkJ.-H. ChoiPRMT1 and PRMT4 regulate oxidative stress-induced retinal pigment epithelial cell damage in SIRT1-dependent and SIRT1-independent mannersOxidative Medicine and Cellular Longevity20152015961791910.1155/2015/6179192-s2.0-84947474496
  106. D. M. DykxhoornC. D. NovinaP. A. SharpKilling the messenger: short RNAs that silence gene expressionNature Reviews Molecular Cell Biology20034645746710.1038/nrm11292-s2.0-003768629512778125
  107. D. P. BartelMicroRNAs: genomics, biogenesis, mechanism, and functionCell2004116228129710.1016/s0092-8674(04)00045-52-s2.0-034744472314744438
  108. A. ZampetakiS. KiechlI. DrozdovPlasma microRNA profiling reveals loss of endothelial miR-126 and other microRNAs in type 2 diabetesCirculation Research2010107681081710.1161/circresaha.110.2263572-s2.0-7795725980320651284
  109. A. R. MurrayQ. ChenY. TakahashiK. K. ZhouK. ParkJ.-X. MaMicroRNA-200b downregulates oxidation resistance 1 (Oxr1) expression in the retina of type 1 diabetes modelInvestigative Ophthalmology & Visual Science20135431689169710.1167/iovs.12-109212-s2.0-8487546980123404117
  110. N. DeyA. BeraF. DasN. Ghosh-ChoudhuryB. S. KasinathG. G. ChoudhuryHigh glucose enhances microRNA-26a to activate mTORC1 for mesangial cell hypertrophy and matrix protein expressionCellular Signalling20152771276128525797045
  111. J. H. SuhE. ChoiM.-J. ChaUp-regulation of miR-26a promotes apoptosis of hypoxic rat neonatal cardiomyocytes by repressing GSK-3β protein expressionBiochemical and Biophysical Research Communications2012423240441010.1016/j.bbrc.2012.05.1382-s2.0-8486284460222664106
  112. Z. ChenT.-P. ShentuL. WenD. A. JohnsonJ. Y.-J. ShyyRegulation of SIRT1 by oxidative stress-responsive miRNAs and a systematic approach to identify its role in the endotheliumAntioxidants & Redox Signaling201319131522153810.1089/ars.2012.48032-s2.0-8488348439923477488
  113. N. D'OnofrioM. VitielloR. CasaleL. ServilloA. GiovaneM. L. BalestrieriSirtuins in vascular diseases: emerging roles and therapeutic potentialBiochimica et Biophysica Acta—Molecular Basis of Disease2015185271311132210.1016/j.bbadis.2015.03.0012-s2.0-84929144042
  114. Y. ColakO. OzturkE. SenatesSIRT1 as a potential therapeutic target for treatment of nonalcoholic fatty liver diseaseMedical Science Monitor2011175HY5HY92-s2.0-7995594131321525818
  115. A. Biason-LauberM. Böni-SchnetzlerB. P. HubbardIdentification of a SIRT1 mutation in a family with type 1 diabetesCell Metabolism201317344845510.1016/j.cmet.2013.02.0012-s2.0-8487533227523473037
  116. E. JingB. EmanuelliM. D. HirscheySirtuin-3 (Sirt3) regulates skeletal muscle metabolism and insulin signaling via altered mitochondrial oxidation and reactive oxygen species productionProceedings of the National Academy of Sciences of the United States of America201110835146081461310.1073/pnas.11113081082-s2.0-8005229118021873205
  117. S. BhattacharyaE. ChaumD. A. JohnsonL. R. JohnsonAge-related susceptibility to apoptosis in human retinal pigment epithelial cells is triggered by disruption of p53-Mdm2 associationInvestigative Ophthalmology & Visual Science201253138350836610.1167/iovs.12-104952-s2.0-8487372646223139272
  118. S. KubotaY. OzawaT. KuriharaRoles of AMP-activated protein kinase in diabetes-induced retinal inflammationInvestigative Ophthalmology and Visual Science201152129142914810.1167/iovs.11-80412-s2.0-8485708908022058332
  119. J.-H. LeeM.-Y. SongE.-K. SongOverexpression of SIRT1 protects pancreatic β-cells against cytokine toxicity by suppressing the nuclear factor-κB signaling pathwayDiabetes200958234435110.2337/db07-17952-s2.0-6324911283619008341
  120. E. M. DioumR. ChenM. S. AlexanderRegulation of hypoxia-inducible factor 2α signaling by the stress-responsive deacetylase sirtuin 1Science200932459321289129310.1126/science.11699562-s2.0-6674912978119498162
  121. Y. ZengK. YangF. WangThe glucagon like peptide 1 analogue, exendin-4, attenuates oxidative stress-induced retinal cell death in early diabetic rats through promoting Sirt1 and Sirt3 expressionExperimental Eye Research201615120321110.1016/j.exer.2016.05.00227212443
  122. R. PazdroJ. R. BurgessThe role of vitamin E and oxidative stress in diabetes complicationsMechanisms of Ageing and Development2010131427628610.1016/j.mad.2010.03.0052-s2.0-7795254870620307566
  123. A. JariyapongskulT. RungjaroenN. KasetsuwanS. PatumrajJ. SekiH. NiimiLong-term effects of oral vitamin C supplementation on the endothelial dysfunction in the iris microvessels of diabetic ratsMicrovascular Research2007741323810.1016/j.mvr.2007.03.0022-s2.0-3424980121117467747
  124. M. KunisakiS.-E. BursellA. C. ClermontVitamin E prevents diabetes-induced abnormal retinal blood flow via the diacylglycerol-protein kinase C pathwayAmerican Journal of Physiology - Endocrinology and Metabolism19952692E239E2462-s2.0-0028895508
  125. N. AlcubierreJ. VallsE. RubinatVitamin D deficiency is associated with the presence and severity of diabetic retinopathy in type 2 diabetes mellitusJournal of Diabetes Research20152015737417810.1155/2015/3741782-s2.0-84930935639
  126. Z. RenW. LiQ. ZhaoL. MaJ. ZhuThe impact of 1,25-dihydroxy vitamin D3 on the expressions of vascular endothelial growth factor and transforming growth factor-β1 in the retinas of rats with diabetesDiabetes Research and Clinical Practice201298347448010.1016/j.diabres.2012.09.0282-s2.0-8487068408123089551
  127. W. WangY. YangW. ZhangW. WuAssociation of tea consumption and the risk of oral cancer: a meta-analysisOral Oncology201450427628110.1016/j.oraloncology.2013.12.0142-s2.0-8489546124324389399
  128. B. KumarS. K. GuptaT. C. NagS. SrivastavaR. SaxenaGreen tea prevents hyperglycemia-induced retinal oxidative stress and inflammation in streptozotocin-induced diabetic ratsOphthalmic Research201247210310810.1159/0003300512-s2.0-8005390136521997135
  129. M. B. GomesC. A. NegratoAlpha-lipoic acid as a pleiotropic compound with potential therapeutic use in diabetes and other chronic diseasesDiabetology & Metabolic Syndrome201461, article 8010.1186/1758-5996-6-802-s2.0-84904774071
  130. S. GolbidiM. BadranI. LaherDiabetes and alpha lipoic acidFrontiers in Pharmacology20112, article 6910.3389/fphar.2011.000692-s2.0-84859479386
  131. J. LinA. BierhausP. BugertEffect of R-(+)-α-lipoic acid on experimental diabetic retinopathyDiabetologia20064951089109610.1007/s00125-006-0174-y2-s2.0-3364641340916520919
  132. H.-P. HammesX. DuD. EdelsteinBenfotiamine blocks three major pathways of hyperglycemic damage and prevents experimental diabetic retinopathyNature Medicine20039329429910.1038/nm8342-s2.0-0344837347
  133. E. BeltramoK. NizheradzeE. BerroneS. TaralloM. PortaThiamine and benfotiamine prevent apoptosis induced by high glucose-conditioned extracellular matrix in human retinal pericytesDiabetes/Metabolism Research and Reviews200925764765610.1002/dmrr.10082-s2.0-7035057854119768736
  134. X. ZhangG. BarileS. ChangApoptosis and cell proliferation in proliferative retinal disorders: PCNA, Ki-67, caspase-3, and PARP expressionCurrent Eye Research200530539540310.1080/027136805909563062-s2.0-2134446816316020270
  135. B. XuJ. ChiuB. FengS. ChenS. ChakrabartiPARP activation and the alteration of vasoactive factors and extracellular matrix protein in retina and kidney in diabetesDiabetes/Metabolism Research and Reviews200824540441210.1002/dmrr.8422-s2.0-4944911124618351623
  136. L. ZhengC. SzabóT. S. KernPoly(ADP-ribose) polymerase is involved in the development of diabetic retinopathy via regulation of nuclear factor-κBDiabetes200453112960296710.2337/diabetes.53.11.29602-s2.0-704422973115504977
  137. H. GotoT. NishikawaK. SonodaEndothelial MnSOD overexpression prevents retinal VEGF expression in diabetic miceBiochemical and Biophysical Research Communications2008366381482010.1016/j.bbrc.2007.12.0412-s2.0-3754906636418083119
  138. R. A. KowluruV. KowluruY. XiongY.-S. HoOverexpression of mitochondrial superoxide dismutase in mice protects the retina from diabetes-induced oxidative stressFree Radical Biology and Medicine20064181191119610.1016/j.freeradbiomed.2006.01.0122-s2.0-3374907095617015165
  139. M. A. B. RosalesK. C. SilvaJ. B. Lopes de FariaJ. M. Lopes de FariaExogenous sod mimetic tempol ameliorates the early retinal changes reestablishing the redox status in diabetic hypertensive ratsInvestigative Ophthalmology & Visual Science20105184327433610.1167/iovs.09-46902-s2.0-7795585894920335612
  140. S. A. MoustafaZinc might protect oxidative changes in the retina and pancreas at the early stage of diabetic ratsToxicology and Applied Pharmacology2004201214915510.1016/j.taap.2004.05.0142-s2.0-844424185715541754
  141. J. KimPericytes and the prevention of diabetic retinopathyDiabetes Research and Clinical Practice200466S49S5110.1016/j.diabres.2003.10.0272-s2.0-964430331815563980
  142. H. P. HammesA. BartmannL. EngelP. WülfrothAntioxidant treatment of experimental diabetic retinopathy in rats with nicanartineDiabetologia199740662963410.1007/s0012500507262-s2.0-00308025269222640
  143. M.-K. BaeS.-H. KimJ.-W. JeongCurcumin inhibits hypoxia-induced angiogenesis via down-regulation of HIF-1Oncology Reports2006156155715622-s2.0-3425017076116685395
  144. T. MrudulaP. SuryanarayanaP. N. B. S. SrinivasG. B. ReddyEffect of curcumin on hyperglycemia-induced vascular endothelial growth factor expression in streptozotocin-induced diabetic rat retinaBiochemical and Biophysical Research Communications2007361252853210.1016/j.bbrc.2007.07.0592-s2.0-3454750513617662242
  145. A.-S. KiangM. M. HumphriesM. CampbellP. HumphriesAntioxidant therapy for retinal diseaseAdvances in Experimental Medicine and Biology201480178378910.1007/978-1-4614-3209-8_982-s2.0-8490480668424664771
  146. M. TanitoH. MasutaniY.-C. KimM. NishikawaA. OhiraJ. YodoiSulforaphane induces thioredoxin through the antioxidant-responsive element and attenuates retinal light damage in miceInvestigative Ophthalmology & Visual Science200546397998710.1167/iovs.04-11202-s2.0-1624437890015728556
  147. J. FanG. XuT. JiangY. QinPharmacologic induction of heme oxygenase-1 plays a protective role in diabetic retinopathy in ratsInvestigative Ophthalmology and Visual Science201253106541655610.1167/iovs.11-92412-s2.0-8487188319422661484
  148. A. S. Arellano-BuendíaM. Tostado-GonzálezF. E. García-ArroyoAnti-inflammatory therapy modulates Nrf2-Keap1 in kidney from rats with diabetesOxidative Medicine and Cellular Longevity2016201611469380110.1155/2016/46938012-s2.0-84959422819
  149. K. P. MaremandaS. KhanG. B. JenaRole of zinc supplementation in testicular and epididymal damages in diabetic rat: involvement of Nrf2, SOD1, and GPX5Biological Trace Element Research2016173245246410.1007/s12011-016-0674-72-s2.0-8496227790727025721
  150. Z. YuW. ShaoY. ChiangOltipraz upregulates the nuclear respiratory factor 2 alpha subunit (NRF2) antioxidant system and prevents insulin resistance and obesity induced by a high-fat diet in C57BL/6J miceDiabetologia201154492293410.1007/s00125-010-2001-82-s2.0-7995381058721161163
  151. S. ZhouJ. JinT. BaiL. R. SachlebenL. CaiY. ZhengPotential drugs which activate nuclear factor E2-related factor 2 signaling to prevent diabetic cardiovascular complications: a focus on fumaric acid estersLife Sciences20151341566210.1016/j.lfs.2015.05.0152-s2.0-8493493323126044512
  152. K.-C. ChoiG. J. MyungY.-H. LeeEpigallocatechin-3-gallate, a histone acetyltransferase inhibitor, inhibits EBV-induced B lymphocyte transformation via suppression of RelA acetylationCancer Research200969258359210.1158/0008-5472.CAN-08-24422-s2.0-5834909474019147572
  153. S. MajidA. A. DarA. E. AhmadBTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancerCarcinogenesis200930466267010.1093/carcin/bgp0422-s2.0-6434911275819221000
  154. E. TiliJ.-J. MichailleB. AdairResveratrol decreases the levels of miR-155 by upregulating miR-663, a microRNA targeting JunB and JunDCarcinogenesis20103191561156610.1093/carcin/bgq1432-s2.0-7795629085820622002
  155. Z. ZhengH. ChenJ. LiSirtuin 1-mediated cellular metabolic memory of high glucose via the LKB1/AMPK/ROS pathway and therapeutic effects of metforminDiabetes201261121722810.2337/db11-04162-s2.0-8455518848822124463
  156. D. A. DuarteM. A. B. RosalesA. PapadimitriouPolyphenol-enriched cocoa protects the diabetic retina from glial reaction through the sirtuin pathwayThe Journal of Nutritional Biochemistry2015261647410.1016/j.jnutbio.2014.09.0032-s2.0-8492624977825448608
  157. S. ZhaoJ. LiN. WangFenofibrate suppresses cellular metabolic memory of high glucose in diabetic retinopathy via a sirtuin 1-dependent signalling pathwayMolecular Medicine Reports20151246112611810.3892/mmr.2015.41642-s2.0-8494047837026238659
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